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Re-exposure to a heroin-paired context activated the vCA1-IL glutamatergic projections. (a) Schematic showing the ABA procedure of context-induced reinstatement of heroin seeking. (b) Number of active, inactive nose-pokes and heroin infusions were recorded daily during heroin SA training in context A. (c) Number of active and inactive nose-pokes was recorded daily during daily extinction training in context B. (d) Number of active and inactive nose-pokes exposure to context A (ABA) or context B (ABB) during reinstatement testing. Two-way ANOVA, * P <0.05 vs inactive nose-pokes, # P <0.05 vs ABB group, n =8 per group. (e) Left, representative images of FG (injected in IL, blue), <t>NeuN</t> (green) <t>and</t> <t>c-Fos</t> (red) labeling in the vCA1 after re-exposure rats to context A or context B. Scale bar, 100 μm. Top right, representative photomicrograph of FG injection site of the IL. Scale bar, 1 mm. Bottom right, number of FG, NeuN, and c-Fos triple-labeled (FG + NeuN + c-Fos) neurons per mm 2 in the vCA1. * P <0.05 vs the ABB group using an unpaired t -test, n =4 per group. (f) Representative images of FG (blue), c-Fos (green), and EAAC1 (red) or GAD67 (red) in the vCA1 of the ABA group. White arrows showing the neurons expressing EAAC1 (left) GAD67 and FG (right), yellow triangles showing the neurons expressing FG, c-Fos, and EAACA1. Scale bar, 50 μm. * P <0.05 vs GAD67 using an unpaired t -test. n =4 per group. Data are depicted as the mean±SEM.
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Re-exposure to a heroin-paired context activated the vCA1-IL glutamatergic projections. (a) Schematic showing the ABA procedure of context-induced reinstatement of heroin seeking. (b) Number of active, inactive nose-pokes and heroin infusions were recorded daily during heroin SA training in context A. (c) Number of active and inactive nose-pokes was recorded daily during daily extinction training in context B. (d) Number of active and inactive nose-pokes exposure to context A (ABA) or context B (ABB) during reinstatement testing. Two-way ANOVA, * P <0.05 vs inactive nose-pokes, # P <0.05 vs ABB group, n =8 per group. (e) Left, representative images of FG (injected in IL, blue), <t>NeuN</t> (green) <t>and</t> <t>c-Fos</t> (red) labeling in the vCA1 after re-exposure rats to context A or context B. Scale bar, 100 μm. Top right, representative photomicrograph of FG injection site of the IL. Scale bar, 1 mm. Bottom right, number of FG, NeuN, and c-Fos triple-labeled (FG + NeuN + c-Fos) neurons per mm 2 in the vCA1. * P <0.05 vs the ABB group using an unpaired t -test, n =4 per group. (f) Representative images of FG (blue), c-Fos (green), and EAAC1 (red) or GAD67 (red) in the vCA1 of the ABA group. White arrows showing the neurons expressing EAAC1 (left) GAD67 and FG (right), yellow triangles showing the neurons expressing FG, c-Fos, and EAACA1. Scale bar, 50 μm. * P <0.05 vs GAD67 using an unpaired t -test. n =4 per group. Data are depicted as the mean±SEM.
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Re-exposure to a heroin-paired context activated the vCA1-IL glutamatergic projections. (a) Schematic showing the ABA procedure of context-induced reinstatement of heroin seeking. (b) Number of active, inactive nose-pokes and heroin infusions were recorded daily during heroin SA training in context A. (c) Number of active and inactive nose-pokes was recorded daily during daily extinction training in context B. (d) Number of active and inactive nose-pokes exposure to context A (ABA) or context B (ABB) during reinstatement testing. Two-way ANOVA, * P <0.05 vs inactive nose-pokes, # P <0.05 vs ABB group, n =8 per group. (e) Left, representative images of FG (injected in IL, blue), <t>NeuN</t> (green) <t>and</t> <t>c-Fos</t> (red) labeling in the vCA1 after re-exposure rats to context A or context B. Scale bar, 100 μm. Top right, representative photomicrograph of FG injection site of the IL. Scale bar, 1 mm. Bottom right, number of FG, NeuN, and c-Fos triple-labeled (FG + NeuN + c-Fos) neurons per mm 2 in the vCA1. * P <0.05 vs the ABB group using an unpaired t -test, n =4 per group. (f) Representative images of FG (blue), c-Fos (green), and EAAC1 (red) or GAD67 (red) in the vCA1 of the ABA group. White arrows showing the neurons expressing EAAC1 (left) GAD67 and FG (right), yellow triangles showing the neurons expressing FG, c-Fos, and EAACA1. Scale bar, 50 μm. * P <0.05 vs GAD67 using an unpaired t -test. n =4 per group. Data are depicted as the mean±SEM.
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Re-exposure to a heroin-paired context activated the vCA1-IL glutamatergic projections. (a) Schematic showing the ABA procedure of context-induced reinstatement of heroin seeking. (b) Number of active, inactive nose-pokes and heroin infusions were recorded daily during heroin SA training in context A. (c) Number of active and inactive nose-pokes was recorded daily during daily extinction training in context B. (d) Number of active and inactive nose-pokes exposure to context A (ABA) or context B (ABB) during reinstatement testing. Two-way ANOVA, * P <0.05 vs inactive nose-pokes, # P <0.05 vs ABB group, n =8 per group. (e) Left, representative images of FG (injected in IL, blue), <t>NeuN</t> (green) <t>and</t> <t>c-Fos</t> (red) labeling in the vCA1 after re-exposure rats to context A or context B. Scale bar, 100 μm. Top right, representative photomicrograph of FG injection site of the IL. Scale bar, 1 mm. Bottom right, number of FG, NeuN, and c-Fos triple-labeled (FG + NeuN + c-Fos) neurons per mm 2 in the vCA1. * P <0.05 vs the ABB group using an unpaired t -test, n =4 per group. (f) Representative images of FG (blue), c-Fos (green), and EAAC1 (red) or GAD67 (red) in the vCA1 of the ABA group. White arrows showing the neurons expressing EAAC1 (left) GAD67 and FG (right), yellow triangles showing the neurons expressing FG, c-Fos, and EAACA1. Scale bar, 50 μm. * P <0.05 vs GAD67 using an unpaired t -test. n =4 per group. Data are depicted as the mean±SEM.
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Re-exposure to a heroin-paired context activated the vCA1-IL glutamatergic projections. (a) Schematic showing the ABA procedure of context-induced reinstatement of heroin seeking. (b) Number of active, inactive nose-pokes and heroin infusions were recorded daily during heroin SA training in context A. (c) Number of active and inactive nose-pokes was recorded daily during daily extinction training in context B. (d) Number of active and inactive nose-pokes exposure to context A (ABA) or context B (ABB) during reinstatement testing. Two-way ANOVA, * P <0.05 vs inactive nose-pokes, # P <0.05 vs ABB group, n =8 per group. (e) Left, representative images of FG (injected in IL, blue), <t>NeuN</t> (green) <t>and</t> <t>c-Fos</t> (red) labeling in the vCA1 after re-exposure rats to context A or context B. Scale bar, 100 μm. Top right, representative photomicrograph of FG injection site of the IL. Scale bar, 1 mm. Bottom right, number of FG, NeuN, and c-Fos triple-labeled (FG + NeuN + c-Fos) neurons per mm 2 in the vCA1. * P <0.05 vs the ABB group using an unpaired t -test, n =4 per group. (f) Representative images of FG (blue), c-Fos (green), and EAAC1 (red) or GAD67 (red) in the vCA1 of the ABA group. White arrows showing the neurons expressing EAAC1 (left) GAD67 and FG (right), yellow triangles showing the neurons expressing FG, c-Fos, and EAACA1. Scale bar, 50 μm. * P <0.05 vs GAD67 using an unpaired t -test. n =4 per group. Data are depicted as the mean±SEM.
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Re-exposure to a heroin-paired context activated the vCA1-IL glutamatergic projections. (a) Schematic showing the ABA procedure of context-induced reinstatement of heroin seeking. (b) Number of active, inactive nose-pokes and heroin infusions were recorded daily during heroin SA training in context A. (c) Number of active and inactive nose-pokes was recorded daily during daily extinction training in context B. (d) Number of active and inactive nose-pokes exposure to context A (ABA) or context B (ABB) during reinstatement testing. Two-way ANOVA, * P <0.05 vs inactive nose-pokes, # P <0.05 vs ABB group, n =8 per group. (e) Left, representative images of FG (injected in IL, blue), <t>NeuN</t> (green) <t>and</t> <t>c-Fos</t> (red) labeling in the vCA1 after re-exposure rats to context A or context B. Scale bar, 100 μm. Top right, representative photomicrograph of FG injection site of the IL. Scale bar, 1 mm. Bottom right, number of FG, NeuN, and c-Fos triple-labeled (FG + NeuN + c-Fos) neurons per mm 2 in the vCA1. * P <0.05 vs the ABB group using an unpaired t -test, n =4 per group. (f) Representative images of FG (blue), c-Fos (green), and EAAC1 (red) or GAD67 (red) in the vCA1 of the ABA group. White arrows showing the neurons expressing EAAC1 (left) GAD67 and FG (right), yellow triangles showing the neurons expressing FG, c-Fos, and EAACA1. Scale bar, 50 μm. * P <0.05 vs GAD67 using an unpaired t -test. n =4 per group. Data are depicted as the mean±SEM.
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Re-exposure to a heroin-paired context activated the vCA1-IL glutamatergic projections. (a) Schematic showing the ABA procedure of context-induced reinstatement of heroin seeking. (b) Number of active, inactive nose-pokes and heroin infusions were recorded daily during heroin SA training in context A. (c) Number of active and inactive nose-pokes was recorded daily during daily extinction training in context B. (d) Number of active and inactive nose-pokes exposure to context A (ABA) or context B (ABB) during reinstatement testing. Two-way ANOVA, * P <0.05 vs inactive nose-pokes, # P <0.05 vs ABB group, n =8 per group. (e) Left, representative images of FG (injected in IL, blue), <t>NeuN</t> (green) <t>and</t> <t>c-Fos</t> (red) labeling in the vCA1 after re-exposure rats to context A or context B. Scale bar, 100 μm. Top right, representative photomicrograph of FG injection site of the IL. Scale bar, 1 mm. Bottom right, number of FG, NeuN, and c-Fos triple-labeled (FG + NeuN + c-Fos) neurons per mm 2 in the vCA1. * P <0.05 vs the ABB group using an unpaired t -test, n =4 per group. (f) Representative images of FG (blue), c-Fos (green), and EAAC1 (red) or GAD67 (red) in the vCA1 of the ABA group. White arrows showing the neurons expressing EAAC1 (left) GAD67 and FG (right), yellow triangles showing the neurons expressing FG, c-Fos, and EAACA1. Scale bar, 50 μm. * P <0.05 vs GAD67 using an unpaired t -test. n =4 per group. Data are depicted as the mean±SEM.
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Re-exposure to a heroin-paired context activated the vCA1-IL glutamatergic projections. (a) Schematic showing the ABA procedure of context-induced reinstatement of heroin seeking. (b) Number of active, inactive nose-pokes and heroin infusions were recorded daily during heroin SA training in context A. (c) Number of active and inactive nose-pokes was recorded daily during daily extinction training in context B. (d) Number of active and inactive nose-pokes exposure to context A (ABA) or context B (ABB) during reinstatement testing. Two-way ANOVA, * P <0.05 vs inactive nose-pokes, # P <0.05 vs ABB group, n =8 per group. (e) Left, representative images of FG (injected in IL, blue), <t>NeuN</t> (green) <t>and</t> <t>c-Fos</t> (red) labeling in the vCA1 after re-exposure rats to context A or context B. Scale bar, 100 μm. Top right, representative photomicrograph of FG injection site of the IL. Scale bar, 1 mm. Bottom right, number of FG, NeuN, and c-Fos triple-labeled (FG + NeuN + c-Fos) neurons per mm 2 in the vCA1. * P <0.05 vs the ABB group using an unpaired t -test, n =4 per group. (f) Representative images of FG (blue), c-Fos (green), and EAAC1 (red) or GAD67 (red) in the vCA1 of the ABA group. White arrows showing the neurons expressing EAAC1 (left) GAD67 and FG (right), yellow triangles showing the neurons expressing FG, c-Fos, and EAACA1. Scale bar, 50 μm. * P <0.05 vs GAD67 using an unpaired t -test. n =4 per group. Data are depicted as the mean±SEM.
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Image Search Results


Re-exposure to a heroin-paired context activated the vCA1-IL glutamatergic projections. (a) Schematic showing the ABA procedure of context-induced reinstatement of heroin seeking. (b) Number of active, inactive nose-pokes and heroin infusions were recorded daily during heroin SA training in context A. (c) Number of active and inactive nose-pokes was recorded daily during daily extinction training in context B. (d) Number of active and inactive nose-pokes exposure to context A (ABA) or context B (ABB) during reinstatement testing. Two-way ANOVA, * P <0.05 vs inactive nose-pokes, # P <0.05 vs ABB group, n =8 per group. (e) Left, representative images of FG (injected in IL, blue), NeuN (green) and c-Fos (red) labeling in the vCA1 after re-exposure rats to context A or context B. Scale bar, 100 μm. Top right, representative photomicrograph of FG injection site of the IL. Scale bar, 1 mm. Bottom right, number of FG, NeuN, and c-Fos triple-labeled (FG + NeuN + c-Fos) neurons per mm 2 in the vCA1. * P <0.05 vs the ABB group using an unpaired t -test, n =4 per group. (f) Representative images of FG (blue), c-Fos (green), and EAAC1 (red) or GAD67 (red) in the vCA1 of the ABA group. White arrows showing the neurons expressing EAAC1 (left) GAD67 and FG (right), yellow triangles showing the neurons expressing FG, c-Fos, and EAACA1. Scale bar, 50 μm. * P <0.05 vs GAD67 using an unpaired t -test. n =4 per group. Data are depicted as the mean±SEM.

Journal: Neuropsychopharmacology

Article Title: Role of Glutamatergic Projections from the Ventral CA1 to Infralimbic Cortex in Context-Induced Reinstatement of Heroin Seeking

doi: 10.1038/npp.2017.279

Figure Lengend Snippet: Re-exposure to a heroin-paired context activated the vCA1-IL glutamatergic projections. (a) Schematic showing the ABA procedure of context-induced reinstatement of heroin seeking. (b) Number of active, inactive nose-pokes and heroin infusions were recorded daily during heroin SA training in context A. (c) Number of active and inactive nose-pokes was recorded daily during daily extinction training in context B. (d) Number of active and inactive nose-pokes exposure to context A (ABA) or context B (ABB) during reinstatement testing. Two-way ANOVA, * P <0.05 vs inactive nose-pokes, # P <0.05 vs ABB group, n =8 per group. (e) Left, representative images of FG (injected in IL, blue), NeuN (green) and c-Fos (red) labeling in the vCA1 after re-exposure rats to context A or context B. Scale bar, 100 μm. Top right, representative photomicrograph of FG injection site of the IL. Scale bar, 1 mm. Bottom right, number of FG, NeuN, and c-Fos triple-labeled (FG + NeuN + c-Fos) neurons per mm 2 in the vCA1. * P <0.05 vs the ABB group using an unpaired t -test, n =4 per group. (f) Representative images of FG (blue), c-Fos (green), and EAAC1 (red) or GAD67 (red) in the vCA1 of the ABA group. White arrows showing the neurons expressing EAAC1 (left) GAD67 and FG (right), yellow triangles showing the neurons expressing FG, c-Fos, and EAACA1. Scale bar, 50 μm. * P <0.05 vs GAD67 using an unpaired t -test. n =4 per group. Data are depicted as the mean±SEM.

Article Snippet: The number of FG, NeuN, and c-Fos triple-positive cells in the ABA group was significantly higher than that in the ABB group (16.797±4.317 vs 2.240±0.571 per mm 2 ; t 6 =3.34, P =0.0156; ).

Techniques: Injection, Labeling, Expressing